- 1). Place the tip of the micropipette just beneath the surface of the liquid so it's barely submerged. Slowly depress the plunger with your thumb until you reach the second stop so that any fluid or air previously in the pipette is expelled.
- 2). Remove the tip of the micropipette from the fluid and release the pressure on the plunger. The pipette tip will now be filled with air.
- 3). Depress the plunger again, but this time, only to the first stop. You'll feel slightly increased pressure on your thumb when you reach the first stop.
- 4). Submerge the tip of the pipette in the fluid so it's just below the surface. Slowly release the pressure on the plunger while keeping the tip below the surface. Do not release it too rapidly or allow the tip to rise above the surface because this will create air bubbles.
- 5). Stop once the plunger is all the way out at the rest position.